Q: How are the antigens arranged on the slide?
A: Please click on the slide schematic for this information. There is an appropriate schematic for each slide type with multiple antigens.

Q: Why is the conjugate called dual-species?
A: It is a blend of anti-human and anti-mouse conjugates. The anti-mouse conjugate is needed for our controls made with mouse anti-sera.

Q: Why are the controls made with mouse anti-sera?
A: It was necessary to use controls of murine origin because a reliable source of high quality human antiserum is not available for these antigens.

Q: The cells on this IFA slide all appear positive. Is this possible?
A: If all the cells are positive this is likely to be some type of an anti-cellular reaction. The percent positive should be approximately the same as seen with the positive control.

Q: When only two or three cells are seen fluorescing is this a positive?
A: No, the percent of positive cells seen should be at least as high as the number of positive cells seen with the positive control at its endpoint.

Q: Why is the positive control for this IFA kit barely positive at the screening dilution?
A: The positive controls are bottled at the screening dilution and can be titered for an endpoint from this dilution. The control should be used in the first well without further dilution.